A Good Kinda Stress?

Ok, so I can't believe I'm saying this, but I am actually on a bit of a high after having a meeting with my supervisor today. Admittedly, most of it has to do with my (hopefully)future supervisor actually appearing excited about me wanting to do my PhD with him, but atleast 33.3333333% of it has to do with my present supervisor. He actually gave me a good road map of what to achieve in the next week inorder to go on and do something else. I keep repeating to myself "I won't let you down coach!" in my mind as I'm going along which is quite weird! So, to give you a general outline of what I'm doing I'll give you the list I generated:
- Western Blot for Bim
- RNA gel to show quality of RNA to put in thesis
- Call Applied Biosystems to ask how I can sequence my product to make sure its Bim (hoping they say I can't cause that'd be one less thing to do!)
- Read spec sheet on 100bp ladder to see why it isn't clear (Vidya - you got any idea why my ladder would not come out right?)
- Run a thesis-quality gel to show my primer and internal control genes are there and working fine
- Run a gel to show there isn't a difference between bim expression in cells that aren't treated with chemo drugs
- Incubate a 4hr Dex treatment, do RNA isolation and cDNA synthesis
- Make sure realtime PCR is optimised for Bim
- Actually understand what the realtime results mean
- Talk to someone about using the realtime machine on weekends.

hehe, I'm tired from just writing it all!!! But like I said, "I won't let you down coach!"

On top of that, I need to do my intro and methods, go shoe shopping for my bridesmaids shoes before a certain future sister-inlaw has a nervous breakdown, sleep, and occasionally see my family and friends. Oh yeah, and obsessively write silly messages on other peoples blogs....

So, this post has lived up to the title of my blog, and with that I say here endeth this insane rambling!